abstract：:Papaya leaf distortion mosaic virus is highly destructive to commercial papaya production. Here, the complete genome sequence was determined for an isolate of papaya leaf distortion mosaic virus, designated PLDMV-DF, infecting the commercialized papaya ringspot virus (PRSV)-resistant transgenic papaya from China. Excl...

journal_title：Acta virologica

authors： Tuo D,Shen W,Yan P,Li Ch,Gao L,Li X,Li H,Zhou P

更新日期：2013-01-01 00:00:00

abstract：:S100P belongs to several members of the S100 family of calcium-binding proteins, associated with malignant phenotype. Altered levels of S100P expression have been described at different stages and types of cancer. Transcriptional regulation involves different pathways activated by glucocorticoids, growth factors and b...

journal_title：Acta virologica

authors： Tóthová V,Gibadulinová A

更新日期：2013-01-01 00:00:00

abstract：:In this study, a major part of genome of the pestivirus isolate 297 from Slovakia, comprising the 7195 nt-long 5΄-UTR-NS3 region was sequenced and analyzed. Conserved cleavage sites between individual viral proteins of this region were determined and the number of amino acids of respective proteins was estimated as fo...

journal_title：Acta virologica

authors： Lešková V,Jacková A,Vlasáková M,Vilček S

更新日期：2013-01-01 00:00:00

abstract：UNLABELLED:Antigenic and genetic typing of pestiviruses isolated from Indian sheep and goats was carried out. Testing of 1777 sheep and 1026 goat blood samples collected between 2004 and 2008 resulted in isolation of twelve pestiviruses, seven from sheep and five from goats. All of them were antigenically typed as bovi...

journal_title：Acta virologica

authors： Mishra N,Pitale SS,Rajukumar K,Prakash A,Behera SP,Nema RK,Dubey SC

更新日期：2012-01-01 00:00:00

abstract：UNLABELLED:DNA vaccines containing the capsid precursor polypeptide P1 gene of foot-and-mouth disease virus (FMDV) alone or combined with the VP22 gene of bovine herpesvirus 1 (BVP22) as molecular adjuvant were constructed and used for immunization of BALB/c mice. The latter were challenged with FMDV and their humoral ...

journal_title：Acta virologica

authors： Yu X,Xiao S,Jiang Y,Fang L,Chen H,Jin M

更新日期：2012-01-01 00:00:00

abstract：KEYWORDS:virus sanitation; in vitro explants; antiviral mechanism; virus longevity. ...

journal_title：Acta virologica

authors： Luvisi A,Panattoni A,Triolo E

更新日期：2012-01-01 00:00:00

abstract：:Enterovirus 70 (EV70) is the causative agent of acute hemorrhagic conjunctivitis (AHC), for which no effective vaccine is available. This study revealed a high reactivity of the N-terminal region of EV70 VP1 (VP1-1) with an anti-EV70 mouse serum. The analysis of overlapping synthetic peptides of VP1-1 identified a B-c...

journal_title：Acta virologica

authors： Park KB,Lim BK,Ye MB,Chung SY,Nam JH

更新日期：2012-01-01 00:00:00

abstract：:Identification of structural protein relationships is likely to be important for virus assembly and anti-WSSV strategies. In this paper, VP90 of white spot syndrome virus (WSSV) was characterized. Temporal-transcription analysis showed that VP90 is expressed in the late stage of WSSV infection. Far-western and matrix-...

journal_title：Acta virologica

authors： Li Q,Liu QH,Huang J

更新日期：2012-01-01 00:00:00

abstract：:Extreme antigenic and genetic heterogeneity of serotype A foot-and-mouth disease virus (FMDV) population has resulted in change of vaccine strains in India twice in the last decade. In such a situation, complete characterization of the vaccine strains is imperative. With regard to the frequent outbreaks of this diseas...

journal_title：Acta virologica

authors： Mohapatra JK,Pawar SS,Tosh C,Subramaniam S,Palsamy R,Sanyal A,Hemadri D,Pattnaik B

更新日期：2011-01-01 00:00:00

abstract：:A panel of 17 monoclonal antibodies (MAbs) against highly pathogenic avian influenza virus (HPAIV) A/Duck/Novosibirsk/56/05 A/H5N1 (subclade 2.2) isolated in Russian Federation was developed. Immunoblot analysis showed that 12 MAbs were specific for the hemagglutinin (HA) and 5 MAbs for nucleoprotein (NP). All anti-HA...

journal_title：Acta virologica

authors： Masalova OV,Klimova RR,Chichev EV,Fediakina IT,Loginova SY,Borisevich SV,Bondarev VP,Deryabin PG,Lvov DK,Kushch AA

更新日期：2011-01-01 00:00:00

abstract：:We attempted to quantify the protective potential of polyclonal IgG antibodies specific to the ectodomain of M2 protein (eM2) of influenza A virus (IAV) against lethal influenza infection of mice. For this purpose, eM2 conjugated with keyhole limpet hemocyanin (KLH) or KLH alone were administered with Freund's adjuvan...

journal_title：Acta virologica

authors： Király J,Varečková E,Mucha V,Kostolanský F

更新日期：2011-01-01 00:00:00

abstract：:Peanut stripe virus (PStV) is one of the most common viruses infecting peanut that causes great economic losses every year. The 3ʹ-terminal 1082 bp of 74 PStV isolates collected from 12 districts of Shandong province, China were sequenced. Their coat protein (CP) genes were 864 bp in length and shared identities of 98...

journal_title：Acta virologica

authors： Hou SS,Chi YC,Liu YY,Li XD,Yu SL,Li XD

更新日期：2011-01-01 00:00:00

abstract：:ORF 29 of Bombyx mori nucleopolyhedrovirus (BmNPV ORF29), a conserved gene among all the lepidopteran baculoviruses, was subjected to analysis of sequence and transcription/translation in the insect BmN cells in vitro. His-tagged BmNPV ORF29 fusion protein was expressed in Escherichia coli, purified and used for the p...

journal_title：Acta virologica

authors： Chen H,Li G,Huang G,Chen K,Yao Q,Guo Z

更新日期：2010-01-01 00:00:00

abstract：:Human respiratory syncytial virus (HRSV) is a leading pathogen causing lower respiratory tract infections in infants and young children worldwide. In line with the development of an effective vaccine against HRSV, a domain of the fusion (F) glycoprotein of HRSV was produced and its immunogenicity and antigenic propert...

journal_title：Acta virologica

authors： Lim SH,Jahanshiri F,Jalilian FA,Rahim RA,Sekawi Z,Yusoff K

更新日期：2010-01-01 00:00:00

abstract：:Nine samples of diseased Malvastrum coromandelianum plants collected from the fields in Yunnan province of China were found to be infected with Malvastrum yellow vein Yunnan virus (MYVYNV), when tested by PCR using specific primers. The results of PCR and Southern blot analysis showed that only 4 samples out of 9 were...

journal_title：Acta virologica

authors： Jiang T,Liu P,Liao BL,Wu JX,Huang CJ

更新日期：2010-01-01 00:00:00

abstract：UNLABELLED:Influenza A (H1N1) virus is an important pathogen that can be transmitted in the enzootic cycle involved in influenza epidemics. In this study, we analyzed natural selection on the 260 sequences of subtype 1 hemagglutinin (HA1) gene of H1N1 viruses isolated from humans and swine in Asia. Nonsynonymous and sy...

journal_title：Acta virologica

authors： Xu Z,Zhou R,Jin M,Chen H

更新日期：2010-01-01 00:00:00

abstract：:One-step real-time RT-PCR assay was developed for quantification of the immediate-early (IE), namely IE1 and IE2 transcripts of Rat cytomegalovirus (RCMV), strain ALL-03 in rat embryonic fibroblast cells (REF). This in-house SYBR Green I based RT-PCR was shown to have higher amplification efficiency and detection limi...

journal_title：Acta virologica

authors： Loh HS,Mohd-Azmi ML

更新日期：2009-01-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection represents a serious public health problem worldwide. Development of new therapeutics against HCV has been hampered by the lack of a small-animal model. Until now, it has been unclear which host factors influence the HCV infection. It was known that human CD81 (hCD81) or low-density l...

journal_title：Acta virologica

authors： Lv LP,Jia SZ,Wang QL,Zhang YY,Zhou H,Xu JB

更新日期：2009-01-01 00:00:00

abstract：:Tomato yellow leaf curl disease causes severe losses in tomato production throughout Mediterranean countries including Tunisia. In order to generate engineered resistance to this disease, an intron-hairpin RNA construct harboring a Tomato yellow leaf curl Sardinia virus (TYLCSV) truncated replication-associated protei...

journal_title：Acta virologica

authors： Ben Tamarzizt H,Gharsallah Chouchane S,Lengliz R,Maxwell DP,Marrakchi M,Fakhfakh H,Gorsane F

更新日期：2009-01-01 00:00:00

abstract：:Human papillomavirus (HPV) E6 and E7 gene products play a central role in the induction of benign proliferation and malignant transformation by interacting with several cellular regulatory proteins such as p53, p16(INK4a), and nuclear factor kappaB (NF-kappaB). In this study, HPV DNA was detected by in situ hybridizat...

journal_title：Acta virologica

authors： Senba M,Buziba N,Mori N,Wada A,Irie S,Toriyama K

更新日期：2009-01-01 00:00:00

abstract：:The immune response of mice injected with influenza vaccine (FluV) or pneumococcal vaccine (PV) given separately or simultaneously was evaluated. Balb/c mice were divided into six groups. Group I served as control, the mice in group II were injected intraperitoneally with PV, in group III intramuscularly with FluV two...

journal_title：Acta virologica

authors： Bessler H,Blay A,Djaldetti M

更新日期：2008-01-01 00:00:00

abstract：:The identification and genetic characterization of bovine viral diarrhea virus (BVDV) isolate 17237 detected in western Slovakia is described. The analysis of 5'-untranslated region (5'-UTR), autoprotease (Npro) gene, and structural genes (C, Erns, E1, E2) was carried out. The percentage of nucleotide and deduced amin...

journal_title：Acta virologica

authors： Novácková M,Jacková A,Kolesárová M,Vilcek S

更新日期：2008-01-01 00:00:00

abstract：:Several putative cell surface receptors have been identified in the Hepatitis C virus (HCV) infection including CD81, low-density lipoprotein (LDL) receptor, scavenger receptor class B type I (SR-BI), and highly sulfated heparan sulfate (HS). In this study, we showed that the binding of soluble heparin to the envelope...

journal_title：Acta virologica

authors： Heo TH

更新日期：2008-01-01 00:00:00

abstract：:To monitor genetic variation between Cucumber mosaic virus (CMV) isolates of northwest Iran, samples of cucurbitaceous plants expressing symptoms similar to those caused by CMV were collected. The samples were first screened by ELISA to detect CMV and to determine its subgroup. All detected CMV isolates appeared to be...

journal_title：Acta virologica

authors： Sokhandan Bashir N,Nematollahi S,Torabi E

更新日期：2008-01-01 00:00:00

abstract：:The RNA-dependent RNA polymerase (RdRp) in viruses of the family Flaviviridae plays an important role in the viral replication process and in the forming of a replicase complex. We used small interfering RNAs (siRNAs) corresponding to the highly conservative Motif V of RdRp gene of different viruses to examine their r...

journal_title：Acta virologica

authors： Xu Z,Li X,Liu R,Si Y,Sun M,Jin M,Chen H,Qian P

更新日期：2007-01-01 00:00:00

abstract：:The seed transmission of the Zucchini yellow mosaic virus (ZYMV) was studied in cucumber using two isolates unrelated in their biological characteristics. Although the virus could be readily detected in mature seeds harvested from infected cucumbers, the seedlings obtained from infected germinated seeds tested negativ...

journal_title：Acta virologica

authors： Glasa M,Kollerova E

更新日期：2007-01-01 00:00:00

abstract：:A 189-bp repeat has been reported previously to be present in the replication origin (ORI) of Human cytomegalovirus (HCMV) Towne strain and to contain essential and supporting sequences (Chen et al., Arch. Virol. 141, 13301 (1996)). In this study, clones of HCMV strain Towne were isolated and analyzed for the copy num...

journal_title：Acta virologica

authors： Chen Z,Watanabe S

更新日期：2007-01-01 00:00:00

abstract：:In the last two decades, preparation of infectious RNA or DNA clones of plant viruses has transformed to a standard laboratory technique, providing an excellent tool for the research of viral gene functions and virus-host interactions. A full-length clone can be transformed in vitro to a viral vector expressing foreig...

journal_title：Acta virologica

authors： Nagyová A,Subr Z

更新日期：2007-01-01 00:00:00

abstract：:Herpes zoster (HZ) is a neurocutaneous disease caused by Varicella-zoster virus (VZV) as a consequence of declined cell-mediated immunity, immune suppression and immunodeficiency. As reactivation of JC polyomavirus (JCPyV) might be linked with immunodeficiency or immunosuppressive therapy, the relationship between HZ ...

journal_title：Acta virologica

authors： Jeong BH,Park SJ,Koo DW,Kim YS

更新日期：2006-01-01 00:00:00

abstract：:Influenza is an ancient disease that has infected humans in irregular intervals throughout recorded history. The most infamous pandemic was "Spanish Flu" which affected large parts of the world population and killed in 1918-1919, at a rough estimate, at least 50 million people. More recently, two influenza A pandemics...

journal_title：Acta virologica

authors： Kollerova E,Betáková T

更新日期：2006-01-01 00:00:00

abstract：:In this study, DNA vaccines consisting of vector IRES-gD expressing Herpes simplex virus 1 (HSV-1) glycoprotein D (gD) and vector IRES-gD-IL-2 coexpressing HSV-1 gD and interleukin-2 (IL-2), respectively, were constructed. After intramuscular inoculation, both vaccines induced in BALB/c mice antibodies as assayed by E...

journal_title：Acta virologica

authors： Li WR,Niu B,Wang JW,Feng ZJ,Wang DX

更新日期：2006-01-01 00:00:00

abstract：:An atypical mild isolate HV5 of Grapevine fanleaf virus (GFLV) was found in a South Moravian viticulture region in Czech Republic. Partial sequence of its RNA2 was determined and compared with available sequences of typical GFLV isolates. Two genomic regions, namely a 814 nt-long one spanning the movement protein (MP)...

journal_title：Acta virologica

authors： Komínek P,Bryxiová M,Glasa M

更新日期：2006-01-01 00:00:00

abstract：:An indirect immunofluorescence assay (IFA) using a recently developed commercial kit for detecting antibodies against Coxiella burnetii (C.b.), the etiological agent of Q fever, has been evaluated using human field serum samples. The IFA was compared with an ELISA and a complement fixation test (CFT). The IFA was base...

journal_title：Acta virologica

authors： Slabá K,Skultéty L,Toman R

更新日期：2005-01-01 00:00:00

abstract：:RNA interference (RNAi) is a biological phenomenon in which introduction of a small, double-stranded interfering RNAs (siRNAs) into a cell causes a specific degradation of homologous single-stranded RNA. siRNA can be delivered into the cell by different approaches including synthetic RNA, in vitro transcribed RNA and ...

journal_title：Acta virologica

authors： Wu K,Gong Y,Zhang X,Zhang Y,Mu Y,Liu F,Song D,Zhu Y,Wu J

更新日期：2005-01-01 00:00:00

abstract：:Two Infectious bursal disease virus (IBDV) isolates, NP1SSH and NP2K were obtained from a severe infectious bursal disease (IBD) outbreak in Nepal in 2002. The hypervariable (HV) region of VP2 gene (1326 bp) of the isolates was generated by RT-PCR and sequenced. The obtained nucleotide sequences were compared with tho...

journal_title：Acta virologica

authors： Sharma K,Hair-Bejo M,Omar AR,Aini I

更新日期：2005-01-01 00:00:00

abstract：:Among diseases transmitted by ticks in the Czech Republic, tick-borne encephalitis (TBE) caused by Tick-borne encephalitis virus (TBEV) and Lyme disease caused by Borrelia burgdorferi spirochete are most important. We propose an effective and specific test for detection of TBEV in a single tick or a pool of ticks base...

journal_title：Acta virologica

authors： Rudenko N,Golovchenko M,Cihlárova V,Grubhoffer L

更新日期：2004-01-01 00:00:00

abstract：:An Indian isolate of Goatpox virus (GTPV) was adapted and propagated in Vero cells for development of an attenuated virus. The virus was initially passaged in primary lamb testes cells and subsequently in Vero cells. At the 55th passage, the virus showed evidence of attenuation when tested for safety in seronegative g...

journal_title：Acta virologica

authors： Hosamani M,Nandi S,Mondal B,Singh RK,Rasool TJ,Bandyopadhyay SK

更新日期：2004-01-01 00:00:00

abstract：:Most adults are asymptomatically infected with Epstein-Barr virus (EBV). Primary EBV infection is commonly associated with acute infectious mononucleosis (IM). T cell immune activation plays an important role in EBV-associated diseases. IM shows a mainly Th1-type profile, so Th1-type cytokines such as interleukin-2 (I...

journal_title：Acta virologica

authors： Corsi MM,Ruscica M,Passoni D,Scarmozzino MG,Gulletta E

更新日期：2004-01-01 00:00:00

abstract：:Studies on interaction between Herpes simplex virus 1 (HSV-1) and human fibroblasts KMB-17 have demonstrated that HSV-1 binding to the cell surface could induce a specific gene response. In this study, the HSV-1 stimulation-related gene 1 (HSRG1), a new so far unknown gene function of cellular response induced by a sp...

journal_title：Acta virologica

authors： Dong S,Dong C,Liu L,Che Y,Sun M,Hu F,Li J,Li Q

更新日期：2003-01-01 00:00:00

abstract：:We followed the viral kinetics and histopathological changes in different organs of immunocompetent mice infected orally with coxsackieviruses B3 (CVB3) Nancy strain and B4 (CVB4) JVB strain separately. The viruses used were not adapted to mouse organs. In the acute phase of infection, the viral kinetics indicated vir...

journal_title：Acta virologica

authors： Bopegamage S,Borsanyiová M,Vargová A,Petrovicová A,Benkovicová M,Gomolcák P

更新日期：2003-01-01 00:00:00